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The role of IgG4 in cutaneous pathology A. Fernandez-Flores IgG4 is an immunoglobulin subtype that has many physiologic and morphologic peculiarities. In cutaneous pathology, IgG4 has been related to the pathogenesis of many diseases.

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Moreover, in the recent years, new IgG4-related diseases have been described. Since some involve the skin, either primarily or as part of their systemic manifestations, we have tried to briefly examine some of the cutaneous conditions related to IgG4. The mandate for a proper preservation in histopathological tissues Maria Comanescu, D. Arsene, Carmen Ardeleanu, G. Bussolati A sequence of technically reproducible procedures is mandatory to guarantee a proper preservation of tissues and to build up the basis for sound diagnoses.

However, while the goal of these procedures was, until recently, to assure only structural histological and cytological preservation, an appropriate preservation of antigenic properties and of nucleic acid integrity is now additionally requested, in order to permit pathologists to provide regign fat burner thermo review biological information necessary for the adoption of personalized therapies.

The present review analyses the sequence of technical steps open to critical variations.

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Passages such as dehydration, paraffin embedding, sectioning and staining are relatively well standardized and allow adoption of dedicated automatic apparatuses, while other pre-analytical steps, i. Our regign fat burner thermo review is involved in European-funded projects tackling these problems with the concrete objective of implementing a model of effective tumors investigations by high performance genetic and molecular methodologies.

The problem of the discrepant quality level of histopathological and cytological preparations involved five European countries and exploiting the potential of "virtual slide technology".

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Concrete issues, techniques and pitfalls, as well as proposed guidelines for processing the tissues are shown in this presentation. Morphology and morphopathology of hypopharyngo-esophageal cancer B. Popescu, C. Moculescu, S. Bertesteanu Cervical esophageal cancer and hypopharyngeal cancer represent a major diagnostic issue in early stages, considering the fact that the implication of both cervical esophageal and hypopharyngeal cancers shows a poor prognostic from the very beginning.

Positive diagnosis can only be made after histopathological analysis and immunohistochemical analysis in addition. The bioptic material is sampled by rigid endoscopy this being the only viable method of assessing data on the tumor prior to the surgery.

Several risk factors influence the biology of this site thus inflicting both cellular and molecular modifications that are the origin of cancer development.

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Natural products locally modulators of the cellular response: therapeutic perspectives in skin burns G. Parvanescu, I. Lascar Local cellular response plays a major role in restoring skin integrity, in burns with infectious complications, chronic fibrous sequelae, etc.

For the study of wound-healing process, different experimental models of skin burn were developed. Mice, rats, rabbits and guinea pigs are the most used laboratory animals, kept under standard conditions of light, temperature, food and water ad libitum. Commonly, by intramuscular injection, general anesthesia was induced with ketamine hydrochloride. Most times, skin burns were inflicted on the dorsal region of animals. Metal devices with different shapes and weights, heated in water at various temperatures were applied locally, for few seconds.

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The paper reports on 65 natural medicinal products recommended for the external and internal treatment of skin damages first- or second-degree burns, various wounds, ulcerations. Some of them are traditionally used in the Romanian ethnopharmacology, supporting the wound-healing process mainly because of their epithelizing, astringent, emollient, demulcent, anti-inflammatory, antimicrobial, immunomodulatory and antioxidant properties. DNA extraction was performed using a MasterPure purification kit.

Results: For all types of cytological specimens, the quantity of isolated DNA was adequate and allowed amplification.

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Histological and immunohistochemical changes of the myocardium in dilated cardiomyopathy R. Radu, Adriana Bold, O. Pop, D. Malaescu, Irina Gheorghisor, L. Mogoanta Dilated cardiomyopathy is a major cause of cura de slabire disociata pe 7 zile failure and a major cause of morbidity and mortality. It is a multifactorial disease that includes both hereditary and acquired forms.

It is the third most common cause of heart failure and the most common cause of heart transplant. Dilated cardiomyopathy can be a secondary condition of many diseases such as coronary heart injecții de accelerație a arzătorului de grăsime, diabetes, pheochromocytoma, infections, malnutrition, ingestion of toxic substances alcohol, cocaineingestion of chemotherapeutic drugs, autoimmune diseases.

In our study, we aimed to describe the changes of myocardial cells and interstitial regign fat burner thermo review tissue in patients clinically diagnosed with alcoholic dilated cardiomyopathy. The material studied consisted of heart fragments sampled from the left ventricle LV during necropsy from a total of 28 patients, aged between 58 and 73 years, with a clinical and laboratory diagnosis of dilated cardiomyopathy, hospitalized in the Cardiology Center of the Emergency County Hospital of Craiova in and In dilated cardiomyopathy, myocardial muscle fibers appeared slightly elongated or wavy, with hypochromatic, heterogeneous, vacuolar sarcoplasm, by a decrease of myofibril numbers.

Lipofuscin granules were frequently seen in the sarcoplasm.

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Nuclear changes were consistent with sarcoplasmic alterations. Changes of the interstitial connective tissue were sometimes extensive and sometimes barely noticeable. The most common alteration of this structure was the onset and development of a mainly perivascular collagen fibrillogenetic process.

Mogoanta, F. Secureanu, A. Olaru, Carmen Neamtu, B. Totolici, M. Ionila, Stefania Craitoiu Colorectal cancer is an important public health problem worldwide, being the fourth most common cancer in men and the third most common in women.

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Colorectal cancer incidence is higher in developing countries due to the prevalence of obesity associated with reduced physical activity. Rectal mucinous carcinomas, especially the "signet ring cell" type, have a worse prognosis compared with other varieties of colorectal carcinomas, being diagnosed in more advanced stage and more prone to lymph node and peritoneal metastases.

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Our study comprised 37 cases with rectal adenocarcinoma with mucinous component operated in the Surgical Clinics of the Emergency County Hospital of Craiova, between and The aim of this study was to evaluate some molecular prognostic factors for rectal mucinous carcinomas namely B-cell lymphoma 2 Bcl-2 and epidermal growth factor receptor EGFRand their correlations with the main morpho-clinical parameters of these patients. Thus, we immunohistochemically assessed the degree of apoptosis of tumor cells in mucinous rectal carcinomas using the Bcl-2 marker, and tumor aggressiveness using the EGFR responsiveness.

The evaluation of tumor aggressiveness in terms of EGFR responsiveness showed a reduced expression in carcinomas correlated regign fat burner thermo review the increase in quantity of the mucinous component. In addition, EGFR reactivity was increased in the tumor invasion front. Corresponding author: Georgeta Afrem, MD; e-mail: amc.

Morphological variability of the renal artery branching pattern: a brief review and an anatomical study Ecaterina Daescu, Delia Elena Zahoi, A. TheTerminologia Anatomica homologates two branches of the renal artery anterior, posterior and five segmental arteries: four from the anterior branch and one from the posterior one.

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The purpose of this study is to evaluate the renal artery branching pattern, the number and origin of the segmental arteries, as well as to review data from similar studies. The study material consisted of 60 formalin-fixed adult kidneys.

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Dissections and microdissections were performed on the renal arteries and their branches. The branching of the renal artery was prehilar in Type I trunks are those that originate directly from the main renal artery.

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They divide either into segmental branches, or into just branches and a smaller trunk type II. Regign fat burner thermo review type II trunks further divide into other segmental branches. These common trunks must be taken into account to regign fat burner thermo review confusion with the segmental arteries.

Knowledge of these variations is useful not only morphologically, but also clinically. Chronic gastritis with intestinal metaplasia: clinico-statistical, histological and immunohistochemical study Rodica Dirnu, F. Secureanu, Carmen Neamtu, B. Totolici, O. Pop, P. Mitrut, D. Malaescu, L. Mogoanta Chronic gastritis has a high incidence in adults, causing progressive destruction of glandular structures, favoring the development of gastric atrophy.

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The association of chronic gastritis with intestinal type metaplasia of gastric mucosa has a regign fat burner thermo review outcome as intestinal metaplasia is regarded as a precancerous lesion.

Metaplasia is common in patients with Helicobacter pylori infection and also heavy smokers. The aim of our study was to evaluate the relationship between chronic gastritis and intestinal metaplasia.

The study was conducted on a total of patients, aged between 5 and 90 years, who presented for dyspeptic disorders in the period and were examined clinically and endoscopically.

During the gastroscopic examination, fragments of gastric mucosa were collected for the histopathological study and for highlighting the H. A diagnosis of gastritis was established in patients, representing approximately Most cases with gastritis were found in people of middle age. Gastritis was present in almost all age groups, from teenagers to the elders.

Of the cases of gastritis, urease test was positive in patients, representing approximately Histopathological examination identified the presence of intestinal metaplasia in PCNA immunohistochemistry revealed that cell proliferation processes are intensified in intestinal metaplasia.

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This study highlights the importance of chronic gastritis, intestinal metaplasia, and H. Cellular immunophenotypes in human embryonic, fetal and adult heart Alina Grigore, D.

Arsene, Fl. Ceausu, Maria Comanescu, Florica Staniceanu, Carmen Ardeleanu The cellular immunoprofile of cardiac dysfunctions and lesions of ischemic etiology are insufficiently studied to date, especially regarding the contribution of non-cardiomyocytic structures.

Aiming to explore this immunoprofile, we used immunohistochemistry applied on embryonic, fetal and adult normal or ischemic myocardium. Regign fat burner thermo review observed a decrease of smooth muscle alpha-actin expression in fetal vs. DDR2 and vimentin, which are present in the interstitial cells and cardiomyocytes of the embryo, fetus and normal adult heart, are absent in the fibrotic scar tissue and cicatricial infarction, the latter expressing smooth muscle alpha-actin and CD This suggested that myofibroblasts and not local fibroblasts that participate in ischemic remodeling.

An EGFR-positive vascular network was better represented in the ischemic heart than in the adult normal one, a fact possibly related to EGFR implication in cardiac ischemic pre- and post-conditioning. Therefore, cardiomyocytes and non-cardiomyocytic cells have an undulating immunoprofile according to the intrauterine life stage or age after birth, and a variable contribution in cardiac lesions, mostly in ischemic ones.